MicroRNA expression profiling on arrays enhanced with locked nucleic acids
نویسنده
چکیده
Here we describe a new array system (miRCURYTM LNA Array) for global microRNA (miRNA) profiling of biological samples. The miRCURY LNA Array system uses locked nucleic acid (LNATM), a high-affinity nucleotide analog, to enhance the array capture probes, giving them high sensitivity and excellent selectivity for miRNA targets. We have simultaneously developed a straightforward method for labeling miRNAs in a sample. As a result, the miRCURY LNA Array system can provide sensitive and accurate profiling of miRNAs while requiring less sample and keeping sample handling steps to a minimum.
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